Archive for the ‘Skin Stem Cells’ Category

By Daniella Walsh on September 04th, 2014

By Daniella Walsh | LB Indy

Leslies stem cell

Janet Dreyer earned a doctorate in molecular biology, but in her 50s enrolled at the Pasadena College of Art and Design and became hooked on art. After a hiatus from both science and art for travel, shes back to art, creating a work that combines her training in both fields, The Stem Cell Scientist.

Dreyers computer generated work came to life at the request of Laguna Beach glass and multi-media artist Leslie Davis, who organized The Art of Stem Cells. The show features conceptual works by 29 artists. Their themes address debilitating diseases and injuries and the work of scientists trying to find cures. The month-long exhibition opens Saturday, Sept. 6, at the Orange County Center for Contemporary Art in Santa Ana.

Dreyer delved into history when she built a mosaic for the show. The work includes references to the regenerating powers of the Egyptian scarab god Khepri, showing him rolling a cell instead of the sun, among other images. I chose the mosaic format because the tiles create a sense of motion reminding me of developing cells, Dreyer said.

The exhibitions opening and closing receptions will not only showcase what results when artists interact with 23 scientists, but also introduce art patrons to researchers and examples of their state-of-the art stem cell pursuits. Half of all proceeds will benefit research at the center, led for the past eight years by Dr. Peter Donovan, to whom the show is dedicated.

With a keen interest in science and particularly stem cell therapy, Davis has forged a connection to UC Irvines Sue & Bill Gross Stem Cell Research Center. But since 2005, Davis twin interests have yielded three other medical related art exhibitions, including one for Mission Hospital.

It was her brainpower that led to pairing center researchers with artists selected on the strength and nature of their work.

More:
Stem Cells Star in Marriage of Art and Science

Los Angeles, California (PRWEB) September 03, 2014

Stem Cell technology is the future; looking younger and better without plastic surgery is here now. Stem Cell Beautys debut product line StemLife is spearheading the current beauty renaissance. Among websites that provide stem cell beauty products, Stem Cell Beauty is in a league of its own.

Science is always advancing, why shouldn't your beauty products? questions Albert Faleski, Director of Operations at StemCellBeauty.com.

Most products on the shelves are outdated, whereas we take a different approach to find a formula that works with your body, reinvigorating your own stem cells to provide actual results.

The science behind StemLife is nothing short of groundbreaking. Its trademarked FixT Technology was achieved through reverse engineering to understand how the body maintains and heals itself with our own endogenous combinations of adult stem cells. With this knowledge they developed a means to mimic the natural stem cell processes in our body. Unlike other beauty brands, StemLife uses specific combinations of stem cell types, each cultured under specific state-dependent conditions, using cell types and states that are ideal for the particular tissue. It then creates a set of molecules from multiple stem cell types that is complete and fully formed, rendering maximum benefit and efficiency. This approach of stem cell skin care is extremely unique.

Other leading stem cell-based beauty companies use simpler technology where one stem cell type is chosen to make their molecules. This one-size fits all approach is not efficient and lacks the complexity of StemLifes FixT technology. Some companies mash the cells without allowing their molecules to fully process, which again leads to underachieving results. Many of the largest companies have made no attempt to use new science to formulate better products, providing their customers with over-priced serums proven to be archaic.

StemLifes cutting edge formula is shaping the future of hair regrowth as well, providing an ultramodern solution to those looking to slow the hands of time. Their most popular product, The Advanced Hair Treatment for Women, is essentially the hidden gem the world has been waiting for.

Its popularity stems back to the fact that it actually works. Faleski explained.

Were not big on gimmicks. We prefer showing our customer actual people who have had actual results with our products. After seeing life-changing hair growth with their own eyes, we are confident new customers will try it and have amazing results of their own. The Advanced Hair Treatment for Women is an incredible product that sells itself.

StemLifes most interesting product to date is the Natural Lash & Brow Lash Extend. This product boasts ingredients that are formulated to generate eyelash growth. In a market where eyelash extensions have been the go-to fix for longer lashes, being able to naturally grow them is a revolutionary concept.

Go here to see the original:
Stem Cell Beauty: The Online Shop Revolutionizing the Beauty Industry

A new research has revealed that three major pigment cell types i.e. black cells, reflective silvery cells, and yellow cells helped in forming the stripes on zebrafish.

The research conducted by Max Planck Institute for Developmental Biology in Tubingen showed that the yellow cells undergo dramatic changes in cell shape to tint the stripe pattern of zebrafish.

First author Prateek Mahalwar said that they were surprised to observe such cell behaviours, which were totally unexpected color pattern formation.

The study revealed that the three cell types reached the skin by completely different routes. A pluripotent cell population situated at the dorsal side of the embryo gave rise to larval yellow cells, which covered the skin of the embryo and began to multiply at the onset of metamorphosis when the fish was about two to three weeks old.

However, the black and silvery cells came from a small set of stem cells, which is associated with nerve nodes located close to the spinal cord in each segment.

Brigitte Walderich, a co-author of the Science paper, explained that they were surprised to discover that the small clusters of fluorescently labelled cells in the embryo, which could be followed during larval and juvenile stages to unravel growth and behaviour of the yellow cells, divided and multiplied as differentiated cells to cover the skin of the fish before the silvery and black cells arrive to form the stripes.

The study is published in journal Science.

(Posted on 29-08-2014)

Information on States of India:

Andaman Nicobar | Andhra Pradesh | Assam | Bihar | Chandigarh | Chhattisgarh | Dadar Nagar Haveli | Daman Diu | Delhi | Goa | Gujarat | Haryana | Himachal Pradesh | Jammu Kashmir | Jharkhand | Karnataka | Kerala | Lakshadweep | Madhya Pradesh | Maharashtra | Manipur | Meghalaya | Mizoram | Nagaland | Orissa | Pondicherry | Punjab | Rajasthan | Sikkim | Tamil Nadu | Tripura | Uttar Pradesh | Uttaranchal | West Bengal

Go here to see the original:
How zebrafish forms its stripes revealed

The zebrafish, a small fresh water fish, owes its name to a striking pattern of blue stripes alternating with golden stripes. Three major pigment cell types, black cells, reflective silvery cells, and yellow cells emerge during growth in the skin of the tiny juvenile fish and arrange as a multilayered mosaic to compose the characteristic colour pattern. While it was known that all three cell types have to interact to form proper stripes, the embryonic origin of the pigment cells that develop the stripes of the adult fish has remained a mystery up to now. Scientists of the Max Planck Institute for Developmental Biology in Tbingen have now discovered how these cells arise and behave to form the 'zebra' pattern. Their work may help to understand the development and evolution of the great diversity of striking patterns in the animal world.

Beauty in the living world amazes poets, philosophers and scientists alike. Nobel prize laureate Christiane Nsslein-Volhard, Director of the Department for Genetics at the Max Planck Institute for Developmental Biology, has long been fascinated by the biology behind the colour patterns displayed by animals. Her group uses zebrafish as a model organism to study the genetic basis of animal development.

New research by Nsslein-Volhard's laboratory published in Science shows that the yellow cells undergo dramatic changes in cell shape to tint the stripe pattern of zebrafish. "We were surprised to observe such cell behaviours, as these were totally unexpected from what we knew about colour pattern formation," says Prateek Mahalwar, first author of the study. The study builds on a previous work from the laboratory, which was published in June this year in Nature Cell Biology (NCB), tracing the cell behaviour of silvery and black cells. Both studies describe diligent experiments to uncover the cellular events during stripe pattern formation. Individual juvenile fish carrying fluorescently labelled pigment cell precursors were imaged every day for up to three weeks to chart out the cellular behaviours. This enabled the scientists to trace the multiplication, migration and spreading of individual cells and their progeny over the entire patterning process of stripe formation in the living and growing animal. "We had to develop a very gentle procedure to be able to observe individual fish repeatedly over long periods of time. So we used a state of the art microscope which allowed us to reduce the adverse effects of fluorescence illumination to a minimum," says Ajeet Singh, first author of the earlier NCB study.

Surprisingly, the analysis revealed that the three cell types reach the skin by completely different routes: A pluripotent cell population situated at the dorsal side of the embryo gives rise to larval yellow cells, which cover the skin of the embryo. These cells begin to multiply at the onset of metamorphosis when the fish is about two to three weeks old. However, the black and silvery cells come from a small set of stem cells associated with nerve nodes located close to the spinal cord in each segment. The black cells reach the skin migrating along the segmental nerves to appear in the stripe region, whereas the silvery cells pass through the longitudinal cleft that separates the musculature and then multiply and spread in the skin.

Brigitte Walderich, a co-author of the Science paper, who performed cell transplantations to trace the origin of yellow cells, explains: "My attempt was to create small clusters of fluorescently labelled cells in the embryo which could be followed during larval and juvenile stages to unravel growth and behaviour of the yellow cells. We were surprised to discover that they divide and multiply as differentiated cells to cover the skin of the fish before the silvery and black cells arrive to form the stripes."

A striking observation is that both the silvery and yellow cells are able to switch cell shape and colour, depending on their location. The yellow cells compact to closely cover the dense silvery cells forming the light stripe, colouring it golden, and acquire a loose stellate shape over the black cells of the stripes. The silvery cells thinly spread over the stripe region, giving it a blue tint. They switch shape again at a distance into the dense form to aggregate, forming a new light stripe. These cell behaviours create a series of alternating light and dark stripes. The precise superposition of the dense form of silvery and yellow cells in the light stripe, and the loose silvery and yellow cells superimposed over the black cells in the stripe cause the striking contrast between the golden and blue coloration of the pattern.

The authors speculate that variations on these cell behaviours could be at play in generating the great diversity of colour patterns in fish. "These findings inform our way of thinking about colour pattern formation in other fish, but also in animals which are not accessible to direct observation during development such as peacocks, tigers and zebras," says Nsslein-Volhard -- wondering how her cats got their stripes.

Story Source:

The above story is based on materials provided by Max-Planck-Gesellschaft. Note: Materials may be edited for content and length.

Read more here:
How the zebrafish gets its stripes: Uncovering how beautiful color patterns can develop in animals

By Ciara Curtin

Jeanne Loring and her Scripps Research Institute colleagues transplanted a set of cells into the spinal cords of mice that had lost use of their hind limbs to multiple sclerosis. As the experimentalists expected, within a week, the mice rejected the cells. But after another week, the mice began to walk.

We thought that they wouldnt do anything, says Loring, who directs theCenter for Regenerative Medicineat Scripps. But as her lab has since shown numerous times, and published in Stem Cell Reports, something that these particular so-called neural precursor cells dobeforethe immune system kicks them out seems to make the mouse better.

The cells Lorings team used are derived from induced pluripotent stem cells, which are mature cells, such as skin cells, that have been coaxed with a combination of chemicals to return to an earlier stage of development.

Induced pluripotent cells, also known as iPS cells, pose a number of opportunities for medicine. For instance, Loring is using iPS cells from Parkinsons disease and multiple sclerosis patients to reconstitute cell types that may be damaged in people with those conditions. She is also using them to test how certain drugs or treatments may affect damaged cells in people with conditions such as autism spectrum disorders.

Loring (front row, center) with the Loring Lab Group at the Center for Regenerative Medicine

Loring says no viable long-term treatments exist for the diseases her team has been working on, including Alzheimers disease, Parkinsons disease, and multiple sclerosis, Thats where the need is, she says.

The neural precursor cells that Loring has been using in the mice with MS are young cells that havent quite gotten to the point of being nerves yet. Only certain types of these cells have such a dramatic Lazarus-like effect on the affected mice, but Lorings team can readily identify them based on DNA analysis.

Even so, theyre not yet ready to treat human MS patients with the approach, she says. First, the researchers want to identify what the cells producea protein, perhaps, or a set of proteinsthat allows the mice to walk.

For other diseases, however, researchers are closer to being ready to transplant working versions of reprogrammed cells into sick people.

More:
Could Reprogrammed Cells Fight 'Untreatable' Diseases?

CARLSBAD, CA--(Marketwired - August 28, 2014) - International Stem Cell Corporation (OTCQB: ISCO) (www.internationalstemcell.com), a California-based biotechnology company developing novel stem cell based therapies and biomedical products, today announced that Executive Vice President Dr. Simon Craw will present a corporate overview of ISCO and its subsidiaries at two upcoming investment conferences.

Rodman and Renshaw 16th Annual Global Investment Conference:

Date:Wednesday, September 10, 2014 Time:11:40 a.m. ET Location:New York Palace Hotel, New York, NY Room:Kennedy I

Conference details:http://www.meetmax.com//sched/event_23003/~public/conference_home.html?event_id=23003

AEGIS CAPITAL Corp. 2014 Healthcare and Technology Conference:Date:Thursday, September 11, 2014 Time:10:45 a.m. PT Location:The Encore at Wynn, Las Vegas, NV

Conference details:http://www.meetmax.com/sched/event_25932/~public/conference_home.html?event_id=25932

Please contact the conference organizers if you have an interest in attending the conference or if you would like to arrange a meeting with International Stem Cell Corporation's management team.

About International Stem Cell Corporation

International Stem Cell Corporation is focused on the therapeutic applications of human parthenogenetic stem cells (hpSCs) and the development and commercialization of cell-based research and cosmetic products. ISCO's core technology, parthenogenesis, results in the creation of pluripotent human stem cells from unfertilized oocytes (eggs) hence avoiding ethical issues associated with the use or destruction of viable human embryos. ISCO scientists have created the first parthenogenetic, homozygous stem cell line that can be a source of therapeutic cells for hundreds of millions of individuals of differing genders, ages and racial background with minimal immune rejection after transplantation. hpSCs offer the potential to create the first true stem cell bank, UniStemCell. ISCO also produces and markets specialized cells and growth media for therapeutic research worldwide through its subsidiary Lifeline Cell Technology (www.lifelinecelltech.com), and stem cell-based skin care products through its subsidiary Lifeline Skin Care (www.lifelineskincare.com). More information is available atwww.internationalstemcell.com.

To receive ongoing corporate communications via email, visit: http://www.b2i.us/irpass.asp?BzID=1468&to=ea&s=0.

Continued here:
International Stem Cell Corporation to Present at Two Upcoming Investment Conferences

MANILA The head of the Catholic Bishops' Conference of the Philippines has a reminder to those taking the ice bucket challenge, which supports research efforts of the Amyotrophic Lateral Sclerosis Association (ALSA).

CBCP president Lingayen-Dagupan Archbishop Socrates Villegas said research on ALS involves the use of stem cells.

''ALS is a degenerative disorder and stem-cells apparently hold out the promise of reversing the death and degeneration of brain cells, in particular,'' Villegas said in a statement.

''Stem cells however are most readily harvested from embryos, and it is in this regard that this type of research is ethically problematic."

Citing the ''Instruction on Respect for Human Life in Its Origin and on the Dignity of Procreation,'' Villegas noted that ''human embryos obtained in vitro are human beings and subjects with rights."

ALS is a progressive neurodegenerative disease that attacks nerve cells and pathways in the brain and spinal cord, which eventually leads to paralysis.

Villegas said the ALS Association said in a statement that ''most stem-cell research in ALS is currently focused on iPS (induced pluripotent stem) cells, which are not burdened with ethical issues."

''We are told that iPS cells are 'induced pluripotent stem cells', stem cells created from skin cells. Such cells would indeed be pluripotent, but would not be embryonic cells,'' the CBCP chief said.

''As such, the ethical objection to the use of embryonic cells, whether harvested from embryos, or obtained through in vitro fertilization, would not arise."

The prelate, however, noted that the ALS Association also admitted that ''iPS cells are used in 'most stem-cell research.'''

Follow this link:
Catholics warned about ice bucket challenge

Stem cells heal pooches in pain MIKE MATHER

NICK REED/Fairfax NZ

HAPPY HOUND: Shiloh with owner Adele Holland. She is a different dog since having stem cell injections to relieve arthritis pain, Holland says.

Three years ago australian shepherd dog Shiloh was diagnosed with a severe case of degenerative arthritis that left her limping slowly towards her deathbed.

As time went on, and to the dismay of her Horotiu family, Shiloh became increasingly stiff, was soon no longer able to jump, could barely walk without pain, and eventually had to be carried outside to the toilet.

But, remarkably, the 10-year-old pet is not only still alive today, she is walking and jumping without a trace of pain.

It's a physical improvement her owner Adele Holland describes as "nothing short of a miracle".

Shiloh's recovery is something dozens of arthritic Waikato dogs have now experienced after stem cell injections, a treatment technique adopted by Hamilton veterinarian practice CareVets.

Veterinarian Ivan Aleksic said Shiloh was the first dog to receive stem cells. His practice had successfully repeated the $2600 treatment on more than 40 dogs with arthritis. He described stem cells as "the body's own repair cells".

"They have the ability to divide and differentiate into many different types of cells based on where they are needed throughout the body. They can divide and turn into tissues such as skin, fat, muscle, bone, cartilage and nerve to name a few.

More:
Stem cell treatment helps arthritic dogs

Released: 19-Aug-2014 11:30 AM EDT Embargo expired: 21-Aug-2014 12:00 PM EDT Source Newsroom: Johns Hopkins Medicine Contact Information

Available for logged-in reporters only

Newswise Johns Hopkins stem cell biologists have found a way to reprogram a patients skin cells into cells that mimic and display many biological features of a rare genetic disorder called familial dysautonomia. The process requires growing the skin cells in a bath of proteins and chemical additives while turning on a gene to produce neural crest cells, which give rise to several adult cell types. The researchers say their work substantially expedites the creation of neural crest cells from any patient with a neural crest-related disorder, a tool that lets physicians and scientists study each patients disorder at the cellular level.

Previously, the same research team produced customized neural crest cells by first reprogramming patient skin cells into induced pluripotent stem (iPS) cells, which are similar to embryonic stem cells in their ability to become any of a broad array of cell types.

Now we can circumvent the iPS cells step, saving seven to nine months of time and labor and producing neural crest cells that are more similar to the familial dysautonomia patients cells, says Gabsang Lee, Ph.D., an assistant professor of neurology at the Institute for Cell Engineering and the studys senior author. A summary of the study will be published online in the journal Cell Stem Cell on Aug. 21.

Neural crest cells appear early in human and other animal prenatal development, and they give rise to many important structures, including most of the nervous system (apart from the brain and spinal cord), the bones of the skull and jaws, and pigment-producing skin cells. Dysfunctional neural crest cells cause familial dysautonomia, which is incurable and can affect nerves ability to regulate emotions, blood pressure and bowel movements. Less than 500 patients worldwide suffer from familial dysautonomia, but dysfunctional neural crest cells can cause other disorders, such as facial malformations and an inability to feel pain.

The challenge for scientists has been the fact that by the time a person is born, very few neural crest cells remain, making it hard to study how they cause the various disorders.

To make patient-specific neural crest cells, the team began with laboratory-grown skin cells that had been genetically modified to respond to the presence of the chemical doxycycline by glowing green and turning on the gene Sox10, which guides cells toward maturation as a neural crest cell.

Testing various combinations of molecular signals and watching for telltale green cells, the team found a regimen that turned 2 percent of the cells green. That combination involved turning on Sox10 while growing the cells on a layer of two different proteins and giving them three chemical additives to rewind their genetic memory and stimulate a protein network important for development.

Analyzing the green cells at the single cell level, the researchers found that they showed gene activity similar to that of other neural crest cells. Moreover, they discovered that 40 percent were quad-potent, or able to become the four cell types typically derived from neural crest cells, while 35 percent were tri-potent and could become three of the four. The cells also migrated to the appropriate locations in chick embryos when implanted early in development.

Read more here:
Biologists Reprogram Skin Cells to Mimic Rare Disease

Durham, NC (PRWEB) August 22, 2014

Human induced pluripotent stem cells (hiPSCs) have great potential in the field of regenerative medicine because they can be coaxed to turn into specific cells; however, the new cells dont always act as anticipated. They sometimes mutate, develop into tumors or produce other negative side effects. But in a new study recently published in STEM CELLS Translational Medicine, researchers appear to have found a way around this, simply by removing the material used to reprogram the stem cell after they have differentiated into the desired cells.

The study, by Ken Igawa, M.D., Ph.D., and his colleagues at Tokyo Medical and Dental University along with a team from Osaka University, could have significant implications both in the clinic and in the lab.

Scientists induce (differentiate) the stem cells to become the desired cells, such as those that make up heart muscle, in the laboratory using a reprogramming transgene that is, a gene taken from one organism and introduced into another using artificial techniques.

We generated hiPSC lines from normal human skin cells using reprogramming transgenes, then we removed the reprogramming material. When we compared the transgene-free cells with those that had residual transgenes, both appeared quite similar, Dr. Igawa explained. However, after the cells differentiation into skin cells, clear differences were observed.

Several types of analyses revealed that the keratinocytes cells that make up 90 percent of the outermost skin layer that emerged from the transgene-free hiPSC lines were more like normal human cells than those coming from the hiPSCs that still contained some reprogramming material.

These results suggest that transgene-free hiPSC lines should be chosen for therapeutic purposes, Dr. Igawa concluded.

Human induced pluripotent stem cell (hiPSC) lines have potential for therapeutics because of the customized cells and organs that can potentially be induced from such cells, Anthony Atala, M.D., editor of STEM CELLS Translational Medicine and director of the Wake Forest Institute for Regenerative Medicine. This study illustrates a potentially powerful approach for creating hiPSCs for clinical use.

-#-

The full article, Removal of Reprogramming Transgenes Improves the Tissue Reconstitution Potential of Keratinocytes Generated From Human Induced Pluripotent Stem Cells, can be accessed at http://stemcellstm.alphamedpress.org/content/early/2014/07/14/sctm.2013-0179.abstract.

Read the rest here:
Removing Programming Material After Inducing Stem Cells Could Improve Their Regeneration Ability

Clearwater, FL (PRWEB) August 20, 2014

Botanica Day Spa has unveiled their first-ever stem cell facial. Utilizing the new and popular stem cell line from Pevonia, the anti-aging treatment effectively targets fine lines and wrinkles and naturally repairs the skin. The spa also recently announced August specials, highlighting the new service by offering clients a complimentary dermaplaning session with the purchase of the stem cell facial.

Pevonia continues to be a global leader in natural, botanical-based skincare and aging solutions. The Stem Cells Phyto-Elite collection, launched just last month, features two unique plant-based stem cell sources for skin repair and the reversal of signs of aging. According to Pevonia, the new line contains a concentration of stem cells that may be up to ten times higher than any other currently available product. Stem cells from the Argan tree work to improve skin elasticity while stem cells from the European Comfrey Root speed up skin cell renewal. Take a few moments to learn more about the science: What is a plant stem cell?

Aging appears in different ways, but commonly its seen in the form of wrinkles, loss of elasticity and progressively thinner skin over time, said Gen Obolensky, owner at Botanica Day Spa and an aesthetician/facialist herself. All of these common signs of aging are the result of our skin producing fewer and fewer new skin cells over time. Age reversal treatments generally target only the symptoms of slower cell turnover, so its very exciting to be able to offer innovative products that work from inside the skin at the root of what causes these signs of aging.

Obolensky recognizes the buzz surrounding stem cell beauty treatments, adding, Botanicas stem cell facial uses the new Stem Cells Phyto-Elite line which offers a wide range of age reversal benefits. It cleanses, exfoliates, tones, hydrates and brightens while stimulating faster skin cell repairall of which is backed by clinical research and testing, conducted by a respected skincare leader.

In addition to their offer of a no-cost dermaplaning with the stem cell facial, Botanica recently announced more August spa specials including:

Botanica expects appointment spots to fill up quickly with this special offer. To schedule your stem cell facial treatment, call 727-441-1711 or book online today!

ABOUT BOTANICA DAY SPA Located in downtown Clearwater, Botanica Day Spa specializes in natural treatments for the body, skin and nails. Recognized in 2013 as first runner-up for Creative Loafings Best of the Bay awards, Botanica was voted first runner-up for Best Day Spa and first place for Best Mani/Pedi. Botanica was also voted Best of Tampa Bay for Brazilian bikini waxing, eyebrow shaping and therapeutic massage by CitySearch in 2010. With an emphasis on the use of organic and natural product lines in a cutting edge beauty services setting, Botanicas staff is comprised of 12 fully licensed aestheticians, nail technicians, massage therapists and makeup artists offering a full menu of luxury spa services. The spa celebrates its twentieth year in business in 2014. For more information, please visit http://www.BotanicaDaySpa.com.

Visit link:
New Stem Cell Facial Unveiled at Botanica Day Spa

18 hours ago We can compare this process to the layers of an onion. Transcription factors are at the heart of process efficiency, while epigenetic factors form the outer layers that protect the mechanism from attacks and environmental change. Credit: Elodie Legrand and Sophie Jarriault

How can a specialized cell change its identity? A team from the Institut de Gntique et de Biologie Molculaire et Cellulaire (CNRS/INSERM/Universit de Strasbourg) investigated a 100% effective natural example of this phenomenon, which is called transdifferentiation. This process, by which some cells lose their characteristics and acquire a new identity, could be more generally involved in tissue or organ regeneration in vertebrates, and is a promising research avenue for regenerative medicine. This study identifies the role of epigenetic factors involved in this conversion, underlines the dynamic nature of the process, and shows the key mechanisms for effective transdifferentiation. This work, conducted in collaboration with the Institut Curie, was published on August 15, 2014 in Science.

Our body is constituted of cells that acquired characteristics during development and that fulfill a precise function in each organ: we call these differentiated cells. Generally cells maintain their specificity until they die, but it has been proven that some cells can change state and acquire new functions. This is rare but is found in many species and is called "transdifferentiation".

The team studied this process in C. elegans, a small transparent nematode, where a rectal cell transforms naturally into a motor neuron. This change from one cell type into another occurs without cell division, by a succession of well defined steps that always lead to the same result. The researchers investigated the factors that make the conversion process so stable.

The team had elucidated the role of several transcription factors in this transdifferentiation. But these new results have shown the role of so-called "epigenetic" factors that can modulate gene expression. Two protein complexes are involved in the mechanism. These enzymes act on a histone and when a mutation changes their action, the transdifferentiation stops and the rectal cell no longer transforms into a neuron.

The researchers observed that the two complexes act at different steps and that their role may change as a function of the transcription factors with which they are associated. These results underline the importance of the correct chain of steps for each of these molecules: the dynamic nature of the transdifferentiation mechanism is essential to its stability.

The respective role of genetic and epigenetic factors in biological processes is a hotly debated subject. This work shows how each of these factors acts in transdifferentiation: transcription factors handle initiation and progress whereas epigenetic factors guarantee the constant result. The study even goes further, showing that under "normal" conditions, the epigenetic factors are incidental (even when they are absent the conversion occurs relatively efficiently) but that they are indispensable when there are environmental stressors. So they have a crucial role in maximizing the mechanism's efficacy and ensuring that it remains stable in the face of external variations.

Transdifferentiation is a phenomenon that is poorly understood. It may be involved in the organ regeneration that we observe in some organisms, for example newts, which can reconstruct their eye lens after injury. These results bring key new information to help us understand how to control this process and may open the path to promising therapies, in particular in the field of regenerative medicine.

Explore further: One step closer to cell reprogramming

More information: Sequential Histone Modifying Activities Determines the Robustness of Transdifferentiation; S. Zuryn, A. Ahier, M. Portoso, E. Redhouse White, M.C. Morin, R. Margueron, S. Jarriault; Science; August 15, 2014.

Continued here:
From rectal cells to neurons: Keys to understanding cell transdifferentiation

August 18, 2014

Image Caption: Melbourne researchers have revealed the critical importance of highly specialized immune cells, called natural killer cells, in killing melanoma cells that have spread to the lungs. These natural killer cells could be harnessed to hunt down and kill cancers that have spread in the body. Dr. Nick Huntington (left), Rebecca Delconte (center) and Dr. Priyanka Sathe led the team from the Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia. Credit: Walter and Eliza Hall Institute of Medical Research

Walter and Eliza Hall Institute of Medical Research

Melbourne researchers have revealed the critical importance of highly specialized immune cells, called natural killer cells, in killing melanoma cells that have spread to the lungs. These natural killer cells could be harnessed to hunt down and kill cancers that have spread in the body.

The team, from the Walter and Eliza Hall Institute, also found natural killer cells were critical to the bodys rejection of donor bone marrow transplants and in the runaway immune response during toxic shock syndrome.

The discoveries came after the team showed that a protein called MCL-1 was crucial for survival of natural killer cells, in research published today in the journal Nature Communications. The discovery will help to determine how natural killer cells can be manipulated to fight cancers and other disorders.

Dr Nick Huntington, Dr Priyanka Sathe and Ms Rebecca Delconte from the Molecular Immunology division said MCL-1 could be a target for boosting or depleting natural killer cell populations to treat disease. Natural killer cells are immune predators, scouring the body in search of foreign invaders such as viruses, and sensing changes in our own cells that are associated with cancer.

Dr Huntington said the team showed natural killer cells were needed to fight off invading tumor cells that had spread past the original cancer site.

We discovered MCL-1 is absolutely essential for keeping natural killer cells alive, Dr Huntington said. Without natural killer cells, the body was unable to destroy melanoma metastases that had spread throughout the body, and the cancers overwhelmed the lungs.

Knowing how important natural killer cells are for detecting and destroying cancer cells as they spread suggests they would be a good target for boosting immune defenses to treat cancer.

See the article here:
Specialized Immune Cells Could Stop Cancer Spread

In a boon to stem cell research and regenerative medicine, scientists at Boston Children's Hospital, the Wyss Institute for Biologically Inspired Engineering at Harvard University and Boston University have created a computer algorithm called CellNet as a "roadmap" for cell and tissue engineering, to ensure that cells engineered in the lab have the same favorable properties as cells in our own bodies. CellNet and its application to stem cell engineering are described in two back-to-back papers in the August 14 issue of the journal Cell.

Scientists around the world are engaged in culturing pluripotent stem cells (capable of forming all the body's tissues) and transforming them into specialized cell types for use in research and regenerative medicine. Available as an Internet resource for any scientist to use, CellNet provides a much needed "quality assurance" measure for this work.

The two papers also clarify uncertainty around which methods are best for stem cell engineering, and should advance the use of cells derived from patient tissues to model disease, test potential drugs and use as treatments. For example, using CellNet, one of the studies unexpectedly found that skin cells can be converted into intestinal cells that were able to reverse colitis in a mouse model.

"To date, there has been no systematic means of assessing the fidelity of cellular engineering -- to determine how closely cells made in a petri dish approximate natural tissues in the body," says George Q. Daley, MD, PhD, Director of the Stem Cell Transplantation Program at Boston Children's and senior investigator on both studies. "CellNet was developed to assess the quality of engineered cells and to identify ways to improve their performance."

Gene Signatures

CellNet applies network biology to discover the complex network of genes that are turned on or off in an engineered cell, known as the cell's Gene Regulatory Network or GRN. It then compares that network to the cell's real-life counterpart in the body, as determined from public genome databases. Through this comparison, researchers can rigorously and reliably assess:

"CellNet will also be a powerful tool to advance synthetic biology -- to engineer cells for specific medical applications," says James Collins, PhD, Core Faculty member at the Wyss Institute and the William F. Warren Distinguished Professor at Boston University, co-senior investigator on one of the studies.

Putting CellNet to the Test

The researchers -- including co-first authors Patrick Cahan, PhD and Samantha Morris, PhD, of Boston Children's, and Hu Li, PhD, of the Mayo Clinic, first used CellNet to assess the quality of eight kinds of cells created in 56 published studies.

In a second study, they applied CellNet's teachings to a recurring question in stem cell biology: Is it feasible to directly convert one specialized cell type to another, bypassing the laborious process of first creating an iPS cell? This study looked at two kinds of directly converted cells: liver cells made from skin cells, and macrophages made from B cells.

Original post:
Tissue development 'roadmap' created to guide stem cell medicine

TO SOME people, the term stem cell may seem kind of taboo. I personally would not want something from animals injected into my system. But Im okay with non-invasive treatments, so I was interested to try out a plant-based stem cell facial.

After cleansing and toning, cotton pads moistened with a clear solution were laid on my eyelids to protect them from a three-minute steaming session. This was followed by a special tool called a scrubber that kind of looks like a computer mouse, but helps to remove dead skin cells and unblock pores without using the rather painful pricking tool.

Next, a rejuvenating gel was applied, followed by the plant-derived stem cell formula. A unique cooling machine was used to massage it into the skin for 10 minutes. Using this machine for cold electrophoresis helps the skin absorb serums and vitamins, without having to use injections. This was great for someone like me, who is wary of invasive treatments. The cooling machine feels like having an ice-cold metal ball massaged on the face; very invigorating, indeed.

Just when I thought my skin already got a lot of pampering, the stem cell was followed by a face mask full of natural vitamins. While it penetrated into my skin, I was given an arm and foot massage, which was nice for further relaxation.

With my combination skin, I looked pretty greasy right afterwards. When I woke up the next day, I didnt see a visible difference in my skin, but it was very smooth and supple to the touch. You may not see instant results with a treatment like this, but its a good treatment to maintain radiance, softness and hydration from beneath the surface of the skin.

This type of facial is not recommended for those with oily or acne-prone skin because the added oiliness may exacerbate problems, but it is ideal for those with dry or mature skin, as it is deeply nourishing and moisturizing. After the first treatment or over time, depending on the condition of your skin, stem cell diminishes fine lines, prevents wrinkles, and promotes cell renewal (a process that slows with age) to give that glowing look that signifies healthy, youthful skin.

I tried out the stem cell facial at Lohas skin and slimming center on Paseo Saturnino, Banilad. Its a more upscale experience here with your own room, as opposed to being in one large room with dividers, in case privacy is an issue for you. All of their machines and products are brought in from Korea and their staff, like my therapist Jennylyn, are highly knowledgeable and know just how much pressure to apply during the treatment. The service, facilities and products used add up to a luxurious treatment session that makes one feel very pampered.

Published in the Sun.Star Cebu newspaper on August 15, 2014.

Go here to see the original:
Trying out a stem cell facial

Contact Information

Available for logged-in reporters only

Newswise Hematopoietic stem cells (HSCs) give rise to all other blood cell types, but their development and how their fate is determined has long remained a mystery. In a paper published online this week in Nature, researchers at the University of California, San Diego School of Medicine elaborate upon a crucial signaling pathway and the role of key proteins, which may help clear the way to generate HSCs from human pluripotent precursors, similar to advances with other kinds of tissue stem cells.

Principal investigator David Traver, PhD, professor in the Department of Cellular and Molecular Medicine, and colleagues focused on the Notch signaling pathway, a system found in all animals and known to be critical to the generation of HSCs in vertebrates. Notch signaling between emitting and receiving cells is key to establishing HSC fate during development, said Traver. What has not been known is where, when and how Notch signal transduction is mediated.

Traver and colleagues discovered that the Notch signal is transduced into HSC precursor cells from signal emitting cells in the somite embryologic tissues that eventually contribute to development of major body structures, such as skeleton, muscle and connective tissues much earlier in the process than previously anticipated.

More specifically, they found that JAM proteins, best known for helping maintain tight junctions between endothelial cells to prevent vascular leakage, were key mediators of Notch signaling. When the researchers caused loss of function in JAM proteins in a zebrafish model, Notch signaling and HSCs were also lost. When they enforced Notch signaling through other means, HSC development was rescued.

To date, it has not been possible to generate HSCs de novo from human pluripotent precursors, like induced pluripotent stem cells, said Traver. This has been due in part to a lack of understanding of the complete set of factors that the embryo uses to make HSCs in vivo. It has also likely been due to not knowing in what order each required factor is needed.

Our studies demonstrate that Notch signaling is required much earlier than previously thought. In fact, it may be one of the earliest determinants of HSC fate. This finding strongly suggests that in vitro approaches to instruct HSC fate from induced pluripotent stem cells must focus on the Notch pathway at early time-points in the process. Our findings have also shown that JAM proteins serve as a sort of co-receptor for Notch signaling in that they are required to maintain close contact between signal-emitting and signal-receiving cells to permit strong activation of Notch in the precursors of HSCs.

The findings may have far-reaching implications for eventual development of hematopoietic stem cell-based therapies for diseases like leukemia and congenital blood disorders. Currently, it is not possible to create HSCs from differentiation of embryonic stem cells or induced pluripotent stem cells pluripotent cells artificially derived from non-pluripotent cells, such as skin cells that are being used in other therapeutic research efforts.

Co-authors include Isao Kobayashi, Jingjing Kobayashi-Sun, Albert D. Kim and Claire Pouget, UC San Diego Department of Cellular and Molecular Medicine; Naonobu Fujita, UC San Diego Section of Cell and Developmental Biology; and Toshio Suda, Keio University, Japan.

See the original post:
New Blood: Tracing the Beginnings of Hematopoietic Stem Cells

6 hours ago Growing stem cells in conditions free of animal material makes them safe for use in humans. Credit: Eraxion/iStock/Thinkstock

Human stem cells produced through genetic reprogramming are beset by safety concerns because current techniques alter the DNA of the stem cells and use material from animals to grow them. Now, A*STAR researchers have developed an efficient approach that produces safe, patient-specific human stem cells.

Human induced pluripotent stem cells have the potential to treat a number of diseases without the ethical issues associated with embryonic stem cells. Pluripotent stem cells can be produced from adult cells by introducing genes that reprogram them. Typically, the stem cells are grown on a layer of mouse cells in solutions (known as media) that contain animal proteinsand therefore, potentially may also carry disease. For such stem cells to be safe for use in humans, they need to be grown in 'xeno-free' conditions, which are devoid of material from other animals.

Andrew Wan and Hong Fang Lu at the A*STAR Institute of Bioengineering and Nanotechnology in Singapore and colleagues set out to develop a new xeno-free system. The researchers carried out the genetic reprogramming of cells on an artificially produced protein substrate rather than mouse cells. They also used media that contained no animal components. The result was more efficient reprogramming than seen with conventional approaches.

"A xeno-free system will eliminate the risk of disease transmission from other species, which is important for regulatory approval," explains Wan. "Yet there have been few studies on cell reprogramming under totally xeno-free conditions."

The researchers went one step further by addressing the problem of cells acquiring alterations to their DNA during reprogramming.

"Incorporation of transgenes into the genome of the cell poses another safety issue, risking unwanted genetic alterations," explains Lu. "In our work, the transgenes were introduced to initiate the reprogramming, but after this they were removed from the cell, leading to transgene-free stem cells."

The researchers demonstrated that after genetic reprogramming and the removal of the added genes, the stem cells could still develop into different cells types. They were even able to induce them to form dopaminergic neurons, the type that degenerates in Parkinson's disease. The conditions in which the stem cells were grown mean that they are suitable for clinical use and can be derived from a patient's own cells, ensuring complete compatibility.

"Regulatory approval for clinical application of stem cells largely depends on the conditions in which the stem cells are derived," says Wan. "We present a workable protocol for the reprogramming of fibroblasts to stem cells that minimizes any potential safety risks."

Explore further: Discovery may make it easier to develop life-saving stem cells

Read this article:
Animal-free reprogramming of adult cells improves safety

HOT SPRINGS | A new proposal to not only save but also enhance the Veterans Affairs hospital in Hot Springs surfaced Monday, and would add not only a medical college but also a medical research component involving the use of stem cells to the facility.

The idea, put forward by an Iowa-based, non-profit corporation, would also be built around treating patients with regenerative therapy, which helps skin grow back.

Bob Krause, president of Veterans National Recover Center, was joined by surgeon Don Swift in Hot Springs to presented the proposal at a press conference Monday morning. Their multi-pronged plan has been submitted for consideration to the VA Black Hills Health Care Systems Environmental Impact Statement.

Our proposal has three main areas, Krause told the small audience that attended the press conference. First, the creation of Battle Mountain College, for the training of doctors in the discipline of osteopathic medicine. Krause noted that by having the additional training, a major first hurdle in the BHHCS proposal to close the Hot Springsan inability to draw doctors to the area would be addressed.

We would also build the Battle Mountain Research Institute, for further research into the regenerative therapies, along with the Battle Mountain Clinic to treat those veterans and others who require this cutting-edge treatment, Krause said.

He added that the proposal stipulated that it is to be considered in its entirety and that if the VA medical center should close, everything is off the table. This proposal is not mutually exclusive of the one presented by Save the VA, he said of the Hot Springs-area group that is fighting to save the hospital from closure by the federal government.

Krause and Swift said that the technology, which was created in Switzerland by the military and is awaiting FDA approval in the United States, utilizes regenerative or restorative cells created from fetal stem cells to jump-start a patients ability to regenerate skin tissue. After the patients own skin begins to grow, the regenerative cells die, Krause said.

He said that submitting the new proposal through the EIS process was important, since the research would need to be conducted on federal property because South Dakota law does not allow stem cell research at this time.

Swift noted that an important part to the regenerative therapy process was access to mineral water to help hydrate the tissue and fight infection. Such water can be found in Hot Springs.

In response to a question, Krause said that he understands that there is a question involving fetal stem cell research. But what is the greater good? he asked. Do we overlook a veteran who has experienced having all of his skin burned away by an [explosion], instead of developing that single cell that could help? Are you going to walk away from that cell?

Read more here:
New idea for VA would bring an educational focus

14 hours ago The red swamp crayfish (Procambarus clarkii) is native to the southeastern United States. This species is a popular model organism for studies of the nervous system, and has been used to study fundamental mechanisms involved in the production of new neurons in the adult brain. Credit: Jeanne Benton

Researchers have strived for years to determine how neurons are produced and integrated into the brain throughout adult life. In an intriguing twist, scientists reporting in the August 11 issue of the Cell Press journal Developmental Cell provide evidence that adult-born neurons are derived from a special type of circulating blood cell produced by the immune system. The findingswhich were made in crayfishsuggest that the immune system may contribute to the development of the unknown role of certain brain diseases in the development of brain and other tissues.

In many adult organisms, including humans, neurons in some parts of the brain are continually replenished. While this process is critical for ongoing health, dysfunctions in the production of new neurons may also contribute to several neurological diseases, including clinical depression and some neurodegenerative disorders. Dr. Barbara Beltz of Wellesley College and her colleagues studied crayfish to understand how new neurons are made in adult organisms. When they marked the cells of one crayfish and used this animal as a blood donor for transfusions into another crayfish, the researchers found that the donor blood cells could generate neurons in the recipient.

"These blood cellscalled hemocyteshave functions similar to certain white blood cells in mammals and are produced by the immune system in a blood-forming organ that is functionally analogous to bone marrow," explains Dr. Beltz. "When these cells are released into the circulation, they are attracted to a specialized region in the brain where stem cells divide, and their descendants develop into functional neurons."

The current work demonstrates that the immune system can produce cells with stem cell properties that can give rise to different types of cells, including both hemocytes and nerve cells. "Our findings in crayfish indicate that the immune system is intimately tied to mechanisms of adult neurogenesis, suggesting a much closer relationship between the immune system and nervous system than has been previously appreciated," says co-author Dr. Irene Sderhll, of Uppsala University in Sweden. The flexibility of these immune cells in producing neurons in adult animals raises the intriguing possibility of the presence of similar types of flexibility in other animals. If further studies demonstrated a similar relationship between the immune system and brain in mammals, the findings would stimulate a new area of research into immune therapies to target neurological diseases.

Explore further: New discovery on early immune system development

More information: Developmental Cell, Benton et al.: "Cells from the immune system generate adult-born neurons in crayfish." http://www.cell.com/developmental-cel 1534-5807(14)00405-5

Journal reference: Developmental Cell

Provided by Cell Press

Researchers at Lund University have shed light on how and when the immune system is formed, raising hope of better understanding various diseases in children, such as leukaemia.

Read the original:
Blood cells are new, unexpected source of neurons in crayfish

By Amy Norton HealthDay Reporter

THURSDAY, Aug. 7, 2014 (HealthDay News) -- In a step toward using stem cells to treat paralysis, scientists were able to use cells from an elderly man's skin to regrow nerve connections in rats with damaged spinal cords.

Reporting in the Aug. 7 online issue of Neuron, researchers say the human stem cells triggered the growth of numerous axons -- the fibers that extend from the body of a neuron (nerve cell) to send electrical impulses to other cells.

Some axons even reached the animals' brains, according to the team led by Dr. Mark Tuszynski, a professor of neurosciences at the University of California, San Diego.

"This degree of growth in axons has not been appreciated before," Tuszynski said. But he cautioned that there is still much to be learned about how the new nerve fibers behave in laboratory animals.

Tuszynski likened the potential for stem-cell-induced axon growth to nuclear fusion. If it's contained, you get energy; if it's not contained, you get an explosion.

"Too much axon growth into the wrong places would be a bad thing," Tuszynski said.

For years, researchers have studied the potential for stem cells to restore functioning nerve connections in people with spinal cord injuries. Stem cells are primitive cells that have the capacity to develop into various types of body tissue. Stem cells can come from embryos or be generated from cells taken from a person.

For their study, Tuszynski's team used so-called induced pluripotent stem cells. They took skin cells from a healthy 86-year-old man and genetically reprogrammed them to become similar to embryonic stem cells.

Those stem cells were then used to create primitive neurons, which the researchers embedded into a special scaffold created with the help of proteins called growth factors. From there, the human neurons were grafted into lab rats with spinal cord injuries.

Read more:
Scientists Inch Closer Toward Using Stem Cells for Spinal Injuries

For the first time, scientists have uncovered new information on how stem cells in the human bowel behave, revealing vital clues about the earliest stages in bowel cancer development and how we may begin to prevent it.

The study, led by Queen May University of London (QMUL) and published today in the journal Cell Reports, discovered how many stem cells exist within the human bowel and how they behave and evolve over time. It was revealed that within a healthy bowel, stem cells are in constant competition with each other for survival and only a certain number of stem cells can exist within one area at a time (referred to as the 'stem cell niche'). However, when investigating stem cells in early tumours, the researchers saw increased numbers of stem cells within each area as well as intensified competition for survival, suggesting a link between stem cell activity and bowel cancer development.

The study involved studying stem cells directly within the human body using a specially developed 'toolkit'. The toolkit worked by measuring random mutations that naturally accrue in aging stem cells. The random mutations recorded how the stem cells had behaved, similarly to how the rings on a tree trunk record how a tree grew over time. The techniques used were unique in that scientists were able to study the human stem cells within their natural environment, giving a much more accurate picture of their behaviour.

Until this research, the stem cell biology of the human bowel has remained largely a mystery. This is because most stem cell research is carried out in mice, and it was uncertain how research findings in mice could be applied to humans. However, the scientists in fact found the stem cell biology of human bowels to have significant similarities to mice bowels. This means researchers can continue investigating stem cell activity within mice with the knowledge it is representative of humans -- hopefully speeding up bowel cancer research.

Importantly, these new research methods can also now be applied to investigate stem cells in other parts of the human body such as skin, prostate, lung and breast, with the aim of accelerating cancer research in these areas too.

Dr Trevor Graham, Lecturer in Tumour Biology and Study Author at Queen Mary University of London, comments: "Unearthing how stem cells behave within the human bowel is a big step forward for stem cell research. Until now, stem cell research was mostly conducted in mice or involved taking the stem cells out of their natural environment, thus distorting their usual behaviour. We now want to use the methods developed in this study to understand how stem cells behave inside bowel cancer, so we can increase our understanding of how bowel cancer grows. This will hopefully shed more light on how we can prevent bowel cancer -- the fourth most common cancer in the UK. We are positive this research lays important foundations for future bowel cancer prevention work, as well as prevention work in other cancers."

Dr Marnix Jansen, Histopathologist and Study Author at Queen Mary University of London, comments: "This study was made possible through the involvement of patients either diagnosed with bowel cancer or born with a tendency to develop bowel cancer. Only by investigating tissues taken directly from patients could we study how bowel cancers develop. Our work underlines the importance of patient involvement in scientific research if we are to tackle bowel cancer and help the greatest number of people."

Story Source:

The above story is based on materials provided by Queen Mary, University of London. Note: Materials may be edited for content and length.

Here is the original post:
Stem cell behavior of human bowel discovered for first time

PUBLIC RELEASE DATE:

7-Aug-2014

Contact: Charli Scouller c.scouller@qmul.ac.uk 020-788-27943 Queen Mary, University of London

For the first time, scientists have uncovered new information on how stem cells in the human bowel behave, revealing vital clues about the earliest stages in bowel cancer development and how we may begin to prevent it.

The study, led by Queen May University of London (QMUL) and published today in the journal Cell Reports, discovered how many stem cells exist within the human bowel and how they behave and evolve over time. It was revealed that within a healthy bowel, stem cells are in constant competition with each other for survival and only a certain number of stem cells can exist within one area at a time (referred to as the 'stem cell niche'). However, when investigating stem cells in early tumours, the researchers saw increased numbers of stem cells within each area as well as intensified competition for survival, suggesting a link between stem cell activity and bowel cancer development.

The study involved studying stem cells directly within the human body using a specially developed 'toolkit'. The toolkit worked by measuring random mutations that naturally accrue in ageing stem cells. The random mutations recorded how the stem cells had behaved, similarly to how the rings on a tree trunk record how a tree grew over time. The techniques used were unique in that scientists were able to study the human stem cells within their natural environment, giving a much more accurate picture of their behaviour.

Until this research, the stem cell biology of the human bowel has remained largely a mystery. This is because most stem cell research is carried out in mice, and it was uncertain how research findings in mice could be applied to humans. However, the scientists in fact found the stem cell biology of human bowels to have significant similarities to mice bowels. This means researchers can continue investigating stem cell activity within mice with the knowledge it is representative of humans - hopefully speeding up bowel cancer research.

Importantly, these new research methods can also now be applied to investigate stem cells in other parts of the human body such as skin, prostate, lung and breast, with the aim of accelerating cancer research in these areas too.

Dr Trevor Graham, Lecturer in Tumour Biology and Study Author at Queen Mary University of London, comments: "Unearthing how stem cells behave within the human bowel is a big step forward for stem cell research. Until now, stem cell research was mostly conducted in mice or involved taking the stem cells out of their natural environment, thus distorting their usual behaviour. We now want to use the methods developed in this study to understand how stem cells behave inside bowel cancer, so we can increase our understanding of how bowel cancer grows. This will hopefully shed more light on how we can prevent bowel cancer the fourth most common cancer in the UK. We are positive this research lays important foundations for future bowel cancer prevention work, as well as prevention work in other cancers."

Dr Marnix Jansen, Histopathologist and Study Author at Queen Mary University of London, comments: "This study was made possible through the involvement of patients either diagnosed with bowel cancer or born with a tendency to develop bowel cancer. Only by investigating tissues taken directly from patients could we study how bowel cancers develop. Our work underlines the importance of patient involvement in scientific research if we are to tackle bowel cancer and help the greatest number of people."

See more here:
Scientists uncover stem cell behavior of human bowel for the first time

Contact Information

Available for logged-in reporters only

Newswise Building upon previous research, scientists at the University of California, San Diego School of Medicine and Veterans Affairs San Diego Healthcare System report that neurons derived from human induced pluripotent stem cells (iPSC) and grafted into rats after a spinal cord injury produced cells with tens of thousands of axons extending virtually the entire length of the animals central nervous system.

Writing in the August 7 early online edition of Neuron, lead scientist Paul Lu, PhD, of the UC San Diego Department of Neurosciences and colleagues said the human iPSC-derived axons extended through the white matter of the injury sites, frequently penetrating adjacent gray matter to form synapses with rat neurons. Similarly, rat motor axons pierced the human iPSC grafts to form their own synapses.

The iPSCs used were developed from a healthy 86-year-old human male.

These findings indicate that intrinsic neuronal mechanisms readily overcome the barriers created by a spinal cord injury to extend many axons over very long distances, and that these capabilities persist even in neurons reprogrammed from very aged human cells, said senior author Mark Tuszynski, MD, PhD, professor of Neurosciences and director of the UC San Diego Center for Neural Repair.

For several years, Tuszynski and colleagues have been steadily chipping away at the notion that a spinal cord injury necessarily results in permanent dysfunction and paralysis. Earlier work has shown that grafted stem cells reprogrammed to become neurons can, in fact, form new, functional circuits across an injury site, with the treated animals experiencing some restored ability to move affected limbs. The new findings underscore the potential of iPSC-based therapy and suggest a host of new studies and questions to be asked, such as whether axons can be guided and how will they develop, function and mature over longer periods of time.

While neural stem cell therapies are already advancing to clinical trials, this research raises cautionary notes about moving to human therapy too quickly, said Tuszynski.

The enormous outgrowth of axons to many regions of the spinal cord and even deeply into the brain raises questions of possible harmful side effects if axons are mistargeted. We also need to learn if the new connections formed by axons are stable over time, and if implanted human neural stem cells are maturing on a human time frame months to years or more rapidly. If maturity is reached on a human time frame, it could take months to years to observe functional benefits or problems in human clinical trials.

In the latest work, Lu, Tuszynski and colleagues converted skin cells from a healthy 86-year-old man into iPSCs, which possess the ability to become almost any kind of cell. The iPSCs were then reprogrammed to become neurons in collaboration with the laboratory of Larry Goldstein, PhD, director of the UC San Diego Sanford Stem Cell Clinical Center. The new human neurons were subsequently embedded in a matrix containing growth factors and grafted into two-week-old spinal cord injuries in rats.

Go here to read the rest:
Dramatic Growth of Grafted Stem Cells in Rat Spinal Cord Injuries

PUBLIC RELEASE DATE:

7-Aug-2014

Contact: Mary Beth O'Leary moleary@cell.com 617-397-2802 Cell Press

While neurons normally fail to regenerate after spinal cord injuries, neurons formed from human induced pluripotent stem cells (iPSCs) that were grafted into rats with such injuries displayed remarkable growth throughout the length of the animals' central nervous system. What's more, the iPSCs were derived from skin cells taken from an 86-year-old man. The results, described in the Cell Press journal Neuron, could open up new possibilities in stimulating neuron growth in humans with spinal cord injuries

"These findings indicate that intrinsic neuronal mechanisms readily overcome the barriers created by a spinal cord injury to extend many axons over very long distances and that these capabilities persist even in neurons reprogrammed from very aged human cells," said senior author Mark Tuszynski, MD, PhD, professor of neurosciences and director of the UC San Diego Center for Neural Repair.

After Dr. Tuszynski and his colleagues converted the skin cells into iPSCs, which can be coaxed to develop into nearly any other cell type, the team reprogrammed the cells to become neurons, embedded them in a matrix containing growth factors, and then grafted them into 2-week-old spinal cord injuries in rats.

Three months later, the team found mature neurons and extensive nerve fiber growth across long distances in the rats' spinal cords, including through the wound tissue and even extending into the brain. Despite numerous connections between the implanted neurons and existing rat neurons, functional recovery of the animals' limbs was not restored. The investigators noted that several iPSC grafts contained scars that may have blocked beneficial effects.

Dr. Tuszynski, along with lead author Paul Lu, PhD, of the UC San Diego Department of Neurosciences, and their collaborators are now working to identify the best way to translate neural stem cell therapies for patients with spinal cord injuries, using grafts derived from the patients' own cells.

###

Neuron, Lu et al.: "Long-Distance Axonal Growth from Human Induced Pluripotent Stem Cells After Spinal Cord Injury."

View original post here:
Human skin cells reprogrammed as neurons regrow in rats with spinal cord injuries

The Dr Hadwen Trust awarded 135,078 to Dr Catherine Wright, a lecturer at the Department of Life Sciences at Glasgow Caledonian University and a member of the Institute for Applied Health Research's Diabetes and Biomedical Sciences research group.

Loading article content

The grant will fund a three-year research programme which will allow the university's skin tissue bank to continue providing human skin tissue and cells that can be used for studies related to diabetes research.

This includes issues such as wound healing, as well as the development of human stem cells - which would help to replace the need for animal experimentation.

Dr Wright said: "The funding will allow us to employ a full-time member of staff to assist the academics to run the tissue bank and develop new types of human cell models that can replace animal experiments."

See the rest here:
Diabetes project is given funding boost