Mar. 2015: We are excited to announceGenome Engineering 3.0,a 2-day practical workshop designed to help you use CRISPR-Cas9 system to perform all different types of genome engineering tasks more effectively in your own work.It will be held from May 8-9, 2015 at the Broad Institute (map) in Cambridge, MA, USA.The workshop is open to all and free. For more details, please visit our workshop website, and if you are ready to sign up, you can register here.

Feb. 2014: Collaboration with Osamu Nureki and Hiroshi Nishimasuat University of Tokyoreveals crystal structure of Cas9 in complex with guide RNA and target DNA.

Dec. 2013: New Genome-scale CRISPR Knock-out (GeCKO) functional genomics screening study inShalem* and Sanjana* et al., Science, 2013and companion website.

July 2013: Based on the specificity analysis performed in Hsu et al., Nature Biotechnology 2013, we have released the CRISPR Design Tool. This tool allows users to search for high specificity SpCas9 target sites within DNA sequences of interest.

Apr. 2012: Have a question or need help with trouble shooting? Please visit our newly updated FAQs and Trouble Shooting Tips page.

Feb. 2013: For highest efficiency in genome targeting applications, please use our new chimeric RNA design with longer tracrRNA hybrid.In the earlier paper we useda shorter chimeric RNA design that is not optimal, now the new longer chimeric RNA design is most effective (better than crRNA:tracrRNA pair).This is verified by other published CRISPR paper as well.

Jan. 2013: Please feel free to post your questions to our CRISPR Discussion Forum.

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CRISPR Genome Engineering Resources | learn, share, and discuss

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