A Stem Cell-Based Therapy for Retinitis Pigmentosa
Visit: http://www.uctv.tv/) Retinitis pigmentosa (RP) is a genetic disease that gradually destroys the light sensing nerve cells, called photoreceptors, loc...

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Orthopedic Stem Cell Therapy From Bone Marrow
Stem Cell therapy derived from bone marrow is the latest in modern orthopedic medicine to help you alleviate severe joint pain, and avoid invasive joint repl...

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Using Stem Cell Therapy to Repair Damaged Tissue from Shoulder Impingement Syndrome
Board Certified Orthopedic Surgeon, Dr. Wade McKenna discusses how Stemnexa stem cell therapy and amniotic tissue product can aid in healing frayed shoulder tissue damage from shoulder ...

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Knee arthritis one year after stem cell therapy by Harry Adelson, N.D.
Frank describes his outcome one year after stem cell therapy for his arthritic knee by Harry Adelson, N.D. http://www.docereclinics.com.

By: Harry Adelson, N.D.

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Florida panther receives Stem Cell Therapy
Florida Panther Gets Stem Cell Therapy at Newman Veterinary Centers in Florida.

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Stem Cell Therapy | Simple way to regrow cartilage
http://www.arthritistreatmentcenter.com Pioneering simple new technique to re-grow damaged cartilage Jo Willey writing in the UK Express reported researchers from the University of Texas Health...

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This article was originally distributed via PRWeb. PRWeb, WorldNow and this Site make no warranties or representations in connection therewith.

We cordially invite everyone to attend a special open house event at the Riordan-McKenna Institute of Regenerative Orthopedics and Stem Cell Therapy (RMI) at 801 E. Southlake Blvd. in Southlake, Texas 76092 on Friday, March 6th from 5:30 pm to 7:30 pm. There will be plenty of food, drink and engaging conversation with world-renowned stem cell scientist, Neil H.Riordan, PhD and board-certified orthopedic surgeon, R. Wade McKenna, DO.

Southlake, Texas (PRWEB) March 05, 2015

RMI specializes in Stemnexa non-surgical treatment of acute and chronic orthopedic conditions such as meniscal tears, ACL injuries, rotator cuff injuries, runners knee, tennis elbow, and joint pain due to degenerative conditions like osteoarthritis. Stemnexa may also be administered during orthopedic surgeries to promote better post-surgical outcomes.

Stemnexa combines the latest, patented scientific advances in nearly pain-free bone marrow harvesting with two complimentary cellular technologies: Bone Marrow Aspirate Concentrate (BMAC) and *AlphaGEMS amniotic tissue product.

BMAC contains a patients own mesenchymal stem cells (MSC,) hematopoietic stem cells (CD34+), growth factors and other progenitor cells. AlphaGEMS is composed of collagens and other structural proteins, which provide a biologic matrix that supports angiogenesis, tissue growth and new collagen during tissue regeneration and repair.

*AlphaGEMS product is harvested from donated amniotic sac tissue after normal healthy births. For more information about AlphaGEMS, please visit: http://www.rmiclinic.com/non-surgical-stem-cell-injections-joint-pain/stemnexa-protocol/

Find out more about RMI in the February edition of Society Life Magazine.

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Riordan-McKenna Institute of Regenerative Orthopedics and Stem Cell Therapy Announces Open House in Southlake, Texas ...

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Sotos syndrome is a congenital syndrome that is characterized by varying degrees of mental retardation and a large head circumference etc. It is known that 90% of Sotos syndrome patients have mutations in the NSD1 gene. This time, an international research group has revealed that mutation in the APC2 gene causes symptoms of Sotos syndrome related to the nervous system, from analyses of the Apc2-knockout mouse. They also showed that the APC2 gene is a crucial downstream gene of the NSD1 gene. The results of this work will be published in the journal Cell Reports on the 5th of March 2015.

Research members in Qatar and Canada found that siblings (a sister and a brother), diagnosed with Sotos-like syndrome, have a mutation in the APC2 gene. Research members in Japan, Prof. Masaharu Noda and Assoc. Prof. Takafumi Shintani, at the National Institute for Basic Biology, National Institutes of Natural Sciences, showed that this mutation in the APC2 gene causes loss of the protein function.

The Japanese team has studied APC2 gene functions using mice for several years. They have already reported that APC2 controls the cytoskeletal dynamics, and that the migration of neurons was abnormal in the brain of the Apc2-knockout mice. In the current study, they showed that the Apc2-knockout mouse exhibited significantly impaired learning and memory abilities, together with an abnormal brain structure as well as head shape. All these phenotypes are similar to symptoms of Sotos syndrome. The research group also showed that APC2 is one of the downstream genes of the NSD1 gene, using primarily cultured neurons and developing mouse embryos.

Prof. Noda said, "The downstream mechanisms of NSD1, the responsible gene for Sotos syndrome, had been unclear for a long time. And there was no suitable model animal for Sotos syndrome research. The Apc2-knockout mouse appears to be a good model for future investigation of Sotos syndrome."

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The above story is based on materials provided by National Institutes of Natural Sciences. Note: Materials may be edited for content and length.

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For the first time, CRISPR-Cas9 gene-editing technology has been employed in a whole organism model to systematically target every gene in the genome. A team of scientists at the Broad Institute and MIT's David H. Koch Institute for Integrative Cancer Research have pioneered the use of this technology to "knock out," or turn off, all genes across the genome systematically in an animal model of cancer, revealing genes involved in tumor evolution and metastasis and paving the way for similar studies in other cell types and diseases. The work appears online March 5 in Cell.

"Genome-scale guide RNA libraries are a powerful screening system, and we're excited to start applying it to study gene function in animal models," said co-senior author Feng Zhang, core member of the Broad Institute of MIT and Harvard, investigator at the McGovern Institute for Brain Research at MIT, and assistant professor in the MIT Departments of Brain and Cognitive Sciences and Biological Engineering. "This study represents a first step toward using Cas9 to identify important genes in cancer and other complex diseases in vivo."

"Tumor evolution is an extremely complex set of processes, or hallmarks, controlled by networks of genes," said co-senior author Phillip Sharp, Institute Professor at the Massachusetts Institute of Technology, board member at the Broad Institute, and member of the Koch Institute. "The in vivo application of gene-editing is a powerful platform for functional genomic discovery, offering a novel means to investigate each step in tumor evolution and identify the genes that regulate these hallmarks."

CRISPR-Cas9 gene-editing technology enables scientists to investigate the role of genes and genetic mutations in human biology and disease. The system can remove the function of genes at the DNA level, versus other genetic perturbations like RNA interference that "knock down" genes at the RNA level. Broad Institute scientists previously performed genome-wide screens using CRISPR-Cas9 technology in cellular models, but that approach does not capture the complex processes at play in a whole organism. For example, for cancer to metastasize, malignant cells must leave the primary tumor, enter blood vessels to travel to a distant site in the body, leave the blood vessels, and thrive in a new environment. Zhang and Sharp teamed up to search for genes involved in metastasis by applying CRISPR-Cas9 technology in a whole animal model.

In the new study, cells from a mouse model of non-small cell lung cancer (NSCLC) were treated with the Broad's pooled library of CRISPR guide RNAs targeting every gene in the mouse genome, known as the "mouse genome-scale CRISPR knockout library A" (mGeCKOa), along with the Cas9 DNA-cutting enzyme. The system introduces mutations into specific genes, disrupting their sequence and preventing the production of proteins from those genes. The approach ensured that in each cell, only a single gene was knocked out, and that all genes in the mouse genome were targeted by the heterogeneous population of cells in culture. The researchers then transplanted the cells into a mouse and found that cells treated with the knockout library formed highly metastatic tumors.

Using next-generation sequencing, the scientists were able to identify which genes were knocked out in the primary tumors and in the metastases, indicating that the genes are likely tumor suppressors that normally inhibit tumor growth but, when knocked out, promote it.

The results highlighted some well-known tumor suppressor genes in human cancer, including Pten, Cdkn2a, and Nf2, but included some genes not previously linked to cancer. Unexpectedly, the screen also implicated several microRNAs -- small RNA segments that are functional in the cell.

More experimental work remains to fully explore the genes and microRNAs uncovered in the screen. Metastatic tumors are rarely biopsied in the clinic, making samples for research scarce, but future inclusion of metastases in cancer sequencing studies will yield more insight on hits from this study.

Researchers can take the same in vivo approach described in the Cell paper to examine the effects of gene over-expression, to screen circulating tumor cells or other cell lines, and to explore other cancer phenotypes, such as cancer stem cells, host-environment interactions, and angiogenesis.

"Our work provides a proof-of-principle in vivo knockout screen for identification of genes regulating different routes and steps of tumor evolution," said Sidi Chen, co-first author and a postdoctoral fellow working in the Sharp lab.

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In vivo CRISPR-Cas9 screen sheds light on cancer metastasis, tumor evolution

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Let #39;s Play The Repopulation - Episode 5 - Genetic Engineering
We play The Repopulation and venture into the wide world of Genetic Engineering. We kill, we collect DNA, we make biomass! Join me (Tigwyk) as we slowly work...

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Let #39;s Play The Repopulation - Episode 6 - More Genetic Engineering
I (Tigwyk) actually manage to clone two different animals and demo the process of crafting "Man #39;s Best Friend". Stay tuned for more gameplay videos! This vid...

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Genetic Engineering Biotechnology

By: Meherul Islam

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Genetic Engineering & Biotechnology - Video

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If anyone had devised a way to create a genetically engineered baby, I figured George Church would know about it.

At his labyrinthine laboratory on the Harvard Medical School campus, you can find researchers giving E. Coli a novel genetic code never seen in nature. Around another bend, others are carrying out a plan to use DNA engineering to resurrect the woolly mammoth. His lab, Church likes to say, is the center of a new technological genesisone in which man rebuilds creation to suit himself.

When I visited the lab last June, Church proposed that I speak to a young postdoctoral scientist named Luhan Yang, a Harvard recruit from Beijing whod been a key player in developing a new, powerful technology for editing DNA called CRISPR-Cas9. With Church, Yang had founded a company called eGenesis to engineer the genomes of pigs and cattle, sliding in beneficial genes and editing away bad ones.

As I listened to Yang, I waited for a chance to ask my real questions: Can any of this be done to human beings? Can we improve the human gene pool? The position of much of mainstream science has been that such meddling would be unsafe, irresponsible, and even impossible. But Yang didnt hesitate. Yes, of course, she said. In fact, the laboratory had a project to determine how it could be achieved. She flipped open her laptop to a PowerPoint slide titled Germline Editing Meeting.

Here it was: a technical proposal to alter human heredity.

Germ line is biologists jargon for the egg and sperm, which combine to form an embryo. By editing the DNA of these cells or the embryo itself, it could be possible to eliminate disease genes and to pass those genetic fixes on to future generations. Such a technology could be used to rid families of scourges like cystic fibrosis. It might also be possible to install genes that offer lifelong protection against infection, Alzheimers, and, Yang told me, maybe the effects of aging. These would be history-making medical advances that could be as important to this century as vaccines were to the last.

The fear is that germ line engineering is a path toward a dystopia of super people and designer babies for those who can afford it.

Thats the promise. The fear is that germ line engineering is a path toward a dystopia of super people and designer babies for those who can afford it. Want a child with blue eyes and blond hair? Why not design a highly intelligent group of people who could be tomorrows leaders and scientists?

Just three years after its initial development, CRISPR technology is already widely used by biologists as a kind of search-and-replace tool to alter DNA, even down to the level of a single letter. Its so precise that its widely expected to turn into a promising new approach for gene therapy treatment in people with devastating illnesses. The idea is that physicians could directly correct a faulty gene, say, in the blood cells of a patient with sickle-cell anemia (see Genome Surgery). But that kind of gene therapy wouldnt affect germ cells, and the changes in the DNA wouldnt get passed to future generations.

In contrast, the genetic changes created by germ line engineering would be passed on, and thats what has always made the idea seem so objectionable. So far, caution and ethical concerns have had the upper hand. A dozen countries, not including the United States, have banned germ line engineering, and scientific societies have unanimously concluded that it would be too risky to do. The European Unions convention on human rights and biomedicine says tampering with the gene pool would be a crime against human dignity and human rights.

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Engineering the Perfect Baby

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When it comes to gene expression -- the process by which our DNA provides the recipe used to direct the synthesis of proteins and other molecules that we need for development and survival -- scientists have so far studied one single gene at a time. A new approach developed by Harvard geneticist George Church, Ph.D., can help uncover how tandem gene circuits dictate life processes, such as the healthy development of tissue or the triggering of a particular disease, and can also be used for directing precision stem cell differentiation for regenerative medicine and growing organ transplants.

The findings, reported by Church and his team of researchers at the Wyss Institute for Biologically Inspired Engineering at Harvard University and Harvard Medical School in Nature Methods, show promise that precision gene therapies could be developed to prevent and treat disease on a highly customizable, personalized level, which is crucial given the fact that diseases develop among diverse pathways among genetically-varied individuals. Wyss Core Faculty member Jim Collins, Ph.D., was also a co-author on the paper. Collins is also the Henri Termeer Professor of Medical Engineering & Science and Professor in the Department of Biological Engineering at the Massachusetts Institute of Technology.

The approach leverages the Cas9 protein, which has already been employed as a Swiss Army knife for genome engineering, in a novel way. The Cas9 protein can be programmed to bind and cleave any desired section of DNA -- but now Church's new approach activates the genes Cas9 binds to rather than cleaving them, triggering them to activate transcription to express or repress desired genetic traits. And by engineering the Cas9 to be fused to a triple-pronged transcription factor, Church and his team can robustly manipulate single or multiple genes to control gene expression.

"In terms of genetic engineering, the more knobs you can twist to exert control over the expression of genetic traits, the better," said Church, a Wyss Core Faculty member who is also Professor of Genetics at Harvard Medical School and Professor of Health Sciences and Technology at Harvard and MIT. "This new work represents a major, entirely new class of knobs that we could use to control multiple genes and therefore influence whether or not specific genetics traits are expressed and to what extent -- we could essentially dial gene expression up or down with great precision."

Such a capability could lead to gene therapies that would mitigate age-related degeneration and the onset of disease; in the study, Church and his team demonstrated the ability to manipulate gene expression in yeast, flies, mouse and human cell cultures.

"We envision using this approach to investigate and create comprehensive libraries that document which gene circuits control a wide range of gene expression," said one of the study's lead authors Alejandro Chavez, Ph.D., Postdoctoral Fellow at the Wyss Institute. Jonathan Schieman, Ph.D, of the Wyss Institute and Harvard Medical School, and Suhani Vora, of the Wyss Institute, Massachusetts Institute of Technology, and Harvard Medical School, are also lead co-authors on the study.

The new Cas9 approach could also potentially target and activate sections of the genome made up of genes that are not directly responsible for transcription, and which previously were poorly understood. These sections, which comprise up to 90% of the genome in humans, have previously been considered to be useless DNA "dark matter" by geneticists. In contrast to translated DNA, which contains recipes of genetic information used to express traits, this DNA dark matter contains transcribed genes which act in mysterious ways, with several of these genes often having influence in tandem.

But now, that DNA dark matter could be accessed using Cas9, allowing scientists to document which non-translated genes can be activated in tandem to influence gene expression. Furthermore, these non-translated genes could also be turned into a docking station of sorts. By using Cas9 to target and bind gene circuits to these sections, scientists could introduce synthetic loops of genes to a genome, therefore triggering entirely new or altered gene expressions.

The ability to manipulate multiple genes in tandem so precisely also has big implications for advancing stem cell engineering for development of transplant organs and regenerative therapies.

"In order to grow organs from stem cells, our understanding of developmental biology needs to increase rapidly," said Church. "This multivariate approach allows us to quickly churn through and analyze large numbers of gene combinations to identify developmental pathways much faster than has been previously capable."

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Activating genes on demand: Possible?

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The number of people dying of cervical cancer in the world is now more than ever before, and health agencies should advise adolescent boys and girls to go in for proper vaccination to prevent them from contracting the disease, said Nobel Laureate Harald zur Hausen.

While delivering a lecture on prevention of cancers linked to infections at the Indian Genetics Congress organised by the department of genetic engineering at SRM University, Kattankulathur, on Wednesday, he said, in cervical cancer, prevention had caused a significant decrease in the number of infections.

In his address, M.S. Swaminathan, founder of M.S. Swaminathan Research Foundation, said there are three major dimensions of hunger calorie deprivation, protein deficiency and micronutrient deficiency.

One way to overcome protein hunger is through a pulses revolution, he said.

P. Sathyanarayanan, president of SRM University, called on the Centre to enhance funding for research and development in genetic engineering.

Trilochan Mohapatra, director, Central Rice Research Institute, Bhubaneswar, received the Lifetime Achievement Award, and Swarup K. Parida and Amit Mitra of the National Institute of Plant Genome Research, New Delhi, received the young genetics researchers award.

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Vaccination is crucial to preventing cervical cancer

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Mutants Genetics Gladiators Campaa Supra Jardines Hiperbolicos
Gracias Por Todos Los Dias Mirar Mis Campaas.

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Class 12 -Biology-Genetics-Lec5-Human Blood Group Genetics
Covers genetics of human blood group system along with precautions for safe blood transfusion and disesase Erythroblastosis Featalis To Purchase Full Pack DVDs Visit http://www.sci4you.com.

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EHJ Today - Genetics of Cardiomyopathies

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Aficionado Mendocino Genetics Nattie G
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Strain Review - Lemon Skunk (DNA Genetics)
Our very first strain review! The Curator made a couple of mistakes. He #39;ll learn from them. One that stands out - he says "indica-dominant" one time when he ...

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Genetics: It #39;s Written On Your Face
The features of your face, just like the color of your eyes, are rooted in your genesbut we still know very little about what portion of the genome makes a ...

By: World Science Festival

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Applied Genetics Technology Corporations Sue Washer: Faces of Technology
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02- Chapter 1- Intro to Genetics- Chapter Concepts
Concepts of Genetics 10th ed. (audiobook)

By: Audio Nick

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Dte genetics
Crack berries kicking ass. Loving the structure of this plant.

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Dte genetics - Video

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Paying for gene therapy are annuities the next big thing
Paying for gene therapy are annuities the next big thing.

By: Reuters-BBC-Aljazeera

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Paying for gene therapy are annuities the next big thing - Video

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