Chamber May Open Window for Treating Spine
Posted: February 16, 2012 at 2:23 am
Assessing and developing treatment for spinal cord injuries has long proved difficult for scientists hampered by a lack of available tools and imaging techniques. Now however, a team of Cornell researchers has developed a method to potentially circumvent this problem by surgically implanting a window into the spinal cord of a mouse, allowing for dynamic and long term imaging at a cellular level.
In an article published in the January issue of Nature Methods, Prof. Chris Schaffer, biomedical engineering, and Matthew Farrar grad designed and inserted a chamber into the backs of mice that enables researchers to view of the cellular interactions in spinal cord injury sites. Working alongside Prof. Joseph Fetcho, neurobiology and behavior, Schaffer and Farrar aimed at identifying ways that researchers could advance the quality of spinal cord injury treatment by developing an improved imaging procedure.
The previous method for observing cells after spinal cord injury was to perform multiple surgeries to image the damaged site. However, according to Farrar, multiple surgeries are harmful to the mice because researchers must repeatedly reopen the skin and risk causing inflammation, increasing the risk of infection and the growth of fibrotic tissue. This makes carrying out multiple surgeries a less than ideal practice.
“The goal here is to be able to gain a better understanding of disease dynamics and to create a platform for the robust evaluation of therapeutic strategies,” Schaffer said. “People have been limited in terms of the tools they had available to study spinal cord injury and to develop strategies that could help injured axons regrow and regain function.”
Axons are like the wires of the nervous system, carrying information between the brain and the rest of the body. The axons in the central nervous system do not spontaneously regenerate in adults. After spinal cord trauma, the damaged ends of the axons degenerate from the injury site.
“In treating spinal cord injury, the first thing you have to do is to get the axons to stop dying back and start growing forward,” Farrar said.
Currently, researchers have two ways of assessing the efficacy of drug therapies designed to repair axonal regrowth after spinal cord injury, Schaffer said. The first is to evaluate behavioral differences between animals treated for spinal cord trauma and uninjured ones. The problem, according to Schaffer, is that the animals used in research respond well to drug therapy in general, so scientists can only observe minute differences.
The other technique is to use histology, or the study of microscopic anatomy of cells. Scientists take tissue samples from animals at different time points after a spinal cord injury. There are two problems with this technique, according to Schaffer: One, researchers cannot watch the same animal over time and examine its dynamic cellular behavior at the injury site. Second, researchers cannot watch for when the axons are growing forward.
“If you consider a bundle of axons in an injured spinal cord, some of them might be dying back, some of them might be growing forward, and some of them might not be injured at all,” Farrar said. “If all I show you is a single picture in time then you can’t know which ones are which and this makes it hard to pick out the overall effect of the drug.”
To solve this issue, Schaffer and Farrar developed a tool that enables imaging of the same axons in the same mouse over time, which allows researchers to determine if an axon is getting shorter or longer. This is crucial because some axons may at first, in response to a drug therapy, begin to grow forward, but with time recede back again, according to Farrar. If the axon is not monitored over time, then one might conclude that the drug was more effective than it really was Farrar said.
After designing the chamber, the team was to induced a very small spinal cord injury in the mice and tagged axons and blood vessels with fluorescent markers. This allowed Farrar and Schaffer to observe the superficially severed axons and their growth behavior.
“The idea that you are going to grow back every axon in someone that has spinal cord injury is probably a long way off. And you probably do not have to. The axons that are most likely to regenerate, I would think, are the ones that sort of most robustly remain in the lesion site. And now we have a way to find them using our chamber,” Schaffer said.
The research team also studied microglia, or the inflammatory cells, of the nervous system while studying of axon regrowth. Schaffer said this part of the experiment confirmed findings by other scientists that microglia can interfere with the ability of axons to grow through an injured region because microglia form what are known as glial scars. After cellular trauma, large numbers of inflammatory cells travel to the injury site, clean up debris and recruit other cells to lay down scar tissue. Some research suggests that scar tissue is not something that axons are able to grow through.
According to Schaffer, scientists disagree about how much inflammatory activity is good. While getting rid of cellular debris is crucial to cell functioning, too much of the microglia to clear the debris can form scar tissue through which axons cannot grow. Schaffer and Farrar’s chamber will allow scientists to better determine how to modulate the invasion of these inflammatory cells.
Schaffer and Farrar said that the next steps for scientists using the chamber they developed will be crucial. “This research was aimed at developing a procedure, an implant and an imaging strategy to be able to see spinal cord injury sites over an extended period,” Schaffer said. “But really that is just the beginning, because now we can begin to look how the milieu of things that are released in a spinal cord injury interacts with each other in a way that seems to favor axonal degeneration.”
Visit link:
Chamber May Open Window for Treating Spine
- Levels of Function in Spinal Cord Injury [Last Updated On: May 13th, 2011] [Originally Added On: May 13th, 2011]
- Car accident causes spinal cord injury [Last Updated On: May 13th, 2011] [Originally Added On: May 13th, 2011]
- Spinal Cord Injury Exercise Recovery C-5 Incomplete [Last Updated On: May 13th, 2011] [Originally Added On: May 13th, 2011]
- NCLEX - MS_Nursing_Review_on_ACUTE SPINAL CORD INJURY [Last Updated On: May 17th, 2011] [Originally Added On: May 17th, 2011]
- Jason- spinal cord injury quadriplegic [Last Updated On: May 20th, 2011] [Originally Added On: May 20th, 2011]
- Spinal Cord Injury Research [Last Updated On: May 21st, 2011] [Originally Added On: May 21st, 2011]
- Fourcross - Spinal Cord Injury Downhill Racing [Last Updated On: May 24th, 2011] [Originally Added On: May 24th, 2011]
- Aging with a Spinal Cord Injury [Last Updated On: May 24th, 2011] [Originally Added On: May 24th, 2011]
- Spinal cord injury - Autonomic Dysreflexia [Last Updated On: May 24th, 2011] [Originally Added On: May 24th, 2011]
- Spinal Cord Injury - Embryonic Stem Cells - Dr. Keirstead [Last Updated On: May 24th, 2011] [Originally Added On: May 24th, 2011]
- Spinal Cord Injury Patient after Stem Cell Treatment - Juan Carlos Murillo [Last Updated On: May 31st, 2011] [Originally Added On: May 31st, 2011]
- Spinal Cord Injury: Progress and Promise in Stem Cell Research [Last Updated On: May 31st, 2011] [Originally Added On: May 31st, 2011]
- Starting A New Life After Spinal Cord Injury [Last Updated On: June 3rd, 2011] [Originally Added On: June 3rd, 2011]
- Spinal Cord Injury patient 10 years after injury after Stem Cell Treatment [Last Updated On: June 6th, 2011] [Originally Added On: June 6th, 2011]
- Spinal Cord Injury, Part 1, by David Bailey [Last Updated On: June 8th, 2011] [Originally Added On: June 8th, 2011]
- First Spinal-Cord Surgery With Stem Cells [Last Updated On: June 10th, 2011] [Originally Added On: June 10th, 2011]
- Stem Cell Therapy for Spinal Cord Injury - Injured Airline Pilot Flies Again [Last Updated On: June 11th, 2011] [Originally Added On: June 11th, 2011]
- My Spinal cord Injury story [Last Updated On: June 12th, 2011] [Originally Added On: June 12th, 2011]
- Spinal Cord Injury Center [Last Updated On: June 13th, 2011] [Originally Added On: June 13th, 2011]
- BioFitness - Spinal Cord Injury [Last Updated On: June 19th, 2011] [Originally Added On: June 19th, 2011]
- William Rader MD - Paralyzed Spinal Cord Injury Patient Walks Again [Last Updated On: June 20th, 2011] [Originally Added On: June 20th, 2011]
- Life After Spinal Cord Injury: Albert's Story [Last Updated On: June 23rd, 2011] [Originally Added On: June 23rd, 2011]
- Pushing Boundaries 2009 Video - Spinal Cord Injury [Last Updated On: July 11th, 2011] [Originally Added On: July 11th, 2011]
- Exercise and Spinal Cord injury [Last Updated On: July 18th, 2011] [Originally Added On: July 18th, 2011]
- spinal cord injury book [Last Updated On: July 20th, 2011] [Originally Added On: July 20th, 2011]
- Spinal Cord Injury [Last Updated On: July 23rd, 2011] [Originally Added On: July 23rd, 2011]
- Spinal Cord Injury - Stem Cell Therapy [Last Updated On: July 25th, 2011] [Originally Added On: July 25th, 2011]
- Spinal Cord Injury Recovery Program in South Florida [Last Updated On: July 26th, 2011] [Originally Added On: July 26th, 2011]
- TB Joshua - HEALING, Enthusiastic Teacher Healed of Spinal Cord Injury [Last Updated On: August 4th, 2011] [Originally Added On: August 4th, 2011]
- Dysphagia in Spinal Cord Injury [Last Updated On: August 9th, 2011] [Originally Added On: August 9th, 2011]
- Cal State LA professor describes lab's progress with spinal cord injury clients [Last Updated On: August 9th, 2011] [Originally Added On: August 9th, 2011]
- Mike H. T-12 Complete Spinal Cord Injury [Last Updated On: August 10th, 2011] [Originally Added On: August 10th, 2011]
- Tina: Regaining Independence [Last Updated On: August 15th, 2011] [Originally Added On: August 15th, 2011]
- The CBS Evening News with Scott Pelley - Spinal cord injury and HS football [Last Updated On: August 15th, 2011] [Originally Added On: August 15th, 2011]
- 11Alive NBC News Spotlights Life After Spinal Cord Injury Program [Last Updated On: August 17th, 2011] [Originally Added On: August 17th, 2011]
- Introduction to Understanding Spinal Cord Injury [Last Updated On: August 19th, 2011] [Originally Added On: August 19th, 2011]
- George Washington Masonic Memorial: Spinal Cord Injury [Last Updated On: August 20th, 2011] [Originally Added On: August 20th, 2011]
- Chesley M. T-4/T-5 Incomplete Spinal Cord Injury [Last Updated On: August 24th, 2011] [Originally Added On: August 24th, 2011]
- Spinal cord injury and HS football [Last Updated On: August 28th, 2011] [Originally Added On: August 28th, 2011]
- Spinal Cord Injury: Thoracic Level [Last Updated On: August 30th, 2011] [Originally Added On: August 30th, 2011]
- Mike H. T-12 Complete Spinal Cord Injury - August 2011 [Last Updated On: September 4th, 2011] [Originally Added On: September 4th, 2011]
- Restoring breathing after spinal cord injury [Last Updated On: September 5th, 2011] [Originally Added On: September 5th, 2011]
- Practical Advice for Coping with Spinal Cord Injury.mov [Last Updated On: September 7th, 2011] [Originally Added On: September 7th, 2011]
- Cannabis for MS [Last Updated On: September 9th, 2011] [Originally Added On: September 9th, 2011]
- Rock Climbing for Patients w/Spinal Cord Injuries w/Paralymp [Last Updated On: September 10th, 2011] [Originally Added On: September 10th, 2011]
- Spinal Cord Injury: High Cervical Level [Last Updated On: September 10th, 2011] [Originally Added On: September 10th, 2011]
- Spinal Cord Injury: Causes, Effects and Classifications [Last Updated On: September 10th, 2011] [Originally Added On: September 10th, 2011]
- The Jordanian association for spinal cord injury care [Last Updated On: September 10th, 2011] [Originally Added On: September 10th, 2011]
- OSU Receives $1.7M for Spinal Cord Injury Research [Last Updated On: September 17th, 2011] [Originally Added On: September 17th, 2011]
- Hunter G. T-6 Complete Spinal Cord Injury [Last Updated On: September 20th, 2011] [Originally Added On: September 20th, 2011]
- How much is a Spinal Cord Injury worth? [Last Updated On: September 29th, 2011] [Originally Added On: September 29th, 2011]
- Christopher "Kit" Bond - Spinal Cord Injury Stem Cell Patient [Last Updated On: October 1st, 2011] [Originally Added On: October 1st, 2011]
- Claire Marsh - Spinal Cord Injury Part One [Last Updated On: October 4th, 2011] [Originally Added On: October 4th, 2011]
- Spinal Cord Injury: Low Cervical Level [Last Updated On: October 4th, 2011] [Originally Added On: October 4th, 2011]
- The Spinal Cord Journey - Stem cell therapy stories from three spinal cord injury patients [Last Updated On: October 5th, 2011] [Originally Added On: October 5th, 2011]
- Paraplegic spinal cord injury Child walks on treadmill using Rifton Pacer gait trainer Mar 2011 [Last Updated On: October 5th, 2011] [Originally Added On: October 5th, 2011]
- Spinal cord injury story [Last Updated On: October 5th, 2011] [Originally Added On: October 5th, 2011]
- Sports Opportunities Abound Even After a Spinal Cord Injury [Last Updated On: October 5th, 2011] [Originally Added On: October 5th, 2011]
- Project Walk Spinal Cord Injury Recovery Pintor, Alessandro [Last Updated On: October 5th, 2011] [Originally Added On: October 5th, 2011]
- Monique C5 Quadriplegic spinal cord injury Pacer gait walker by Rifton April 2011 [Last Updated On: October 6th, 2011] [Originally Added On: October 6th, 2011]
- Holly Pediatric paraplegic child spinal cord injury recovery exercises Jan 2011 [Last Updated On: October 7th, 2011] [Originally Added On: October 7th, 2011]
- Walking after Spinal Cord injury and Stem Cells - Video [Last Updated On: October 13th, 2011] [Originally Added On: October 13th, 2011]
- Spinal Cord Injury Margaret's House Modifications.wmv - Video [Last Updated On: October 13th, 2011] [Originally Added On: October 13th, 2011]
- Spinal Cord Injury: Progress and Promise in Stem Cell Research - Video [Last Updated On: October 13th, 2011] [Originally Added On: October 13th, 2011]
- Teen Makes Inspiring Recovery From Spinal Cord Injury - Video [Last Updated On: October 13th, 2011] [Originally Added On: October 13th, 2011]
- Spinal Cord Injury Rehabiliation Success Story - Video [Last Updated On: October 14th, 2011] [Originally Added On: October 14th, 2011]
- April Cave Spinal Cord Injury Recovery.flv - Video [Last Updated On: October 14th, 2011] [Originally Added On: October 14th, 2011]
- L300 Foot Drop System - Testimonials - Persons with Spinal Cord Injury - Video [Last Updated On: October 14th, 2011] [Originally Added On: October 14th, 2011]
- Acetyl l-carnitine Speeds Spinal Cord Injury Recovery by Len Saputo, MD - Video [Last Updated On: October 15th, 2011] [Originally Added On: October 15th, 2011]
- Care for catastrophic injuries, spinal cord and traumatic brain injury - Video [Last Updated On: October 15th, 2011] [Originally Added On: October 15th, 2011]
- Keeping Kids Healthy- Triumph I: Snowboarder Conquers Spinal Cord Injury - Video [Last Updated On: October 16th, 2011] [Originally Added On: October 16th, 2011]
- FSWC SPINAL CORD INJURY CENTRE - Video [Last Updated On: October 18th, 2011] [Originally Added On: October 18th, 2011]
- Spinal cord injury walking in PT - Video [Last Updated On: October 18th, 2011] [Originally Added On: October 18th, 2011]
- Grant to benefit spinal cord injury research - Video [Last Updated On: October 22nd, 2011] [Originally Added On: October 22nd, 2011]
- Mark C5-C7 Quadriplegic spinal cord injury takes un assisted steps. 2011. Congratulations! - Video [Last Updated On: October 23rd, 2011] [Originally Added On: October 23rd, 2011]
- Joe C. C-3/C-4 Spinal Cord Injury-Sit to Stands - Video [Last Updated On: October 23rd, 2011] [Originally Added On: October 23rd, 2011]
- Physical and Mental Adjustments After a Spinal Cord Injury - Video [Last Updated On: October 23rd, 2011] [Originally Added On: October 23rd, 2011]
- "Jenn McCallson", "Project Walk Spinal Cord Injury Recovery" - Video [Last Updated On: October 26th, 2011] [Originally Added On: October 26th, 2011]
- spinal cord injuries - Video [Last Updated On: October 27th, 2011] [Originally Added On: October 27th, 2011]
- Spinal Cord Injury - Video [Last Updated On: October 28th, 2011] [Originally Added On: October 28th, 2011]